Dhiman Sarkar

Dhiman Sarkar

Organic Chemistry Division

About Me

My research group focuses on the development of NCE against infectious diseases with particular emphasis on tuberculosis. Our expertise involves in developing new protocols for high throughput screening of synthetic as well as natural product inhibitors against novel targets as well as whole cells; study the mode of action of inhibitors, mechanism of enzyme-inhibitor interaction. Our major interest is to understand the latent stage of the tuberculosis bacilli and its reactivation into actively growing stage. For these we are using biochemical, proteomics and molecular biology approach on different in vitro dormancy models. We also do screening against different mammalian cell lines to identify anti-proliferative, anti-apoptotic, cytotoxic activities of inhibitors.

Professional Experience

Predoctoral  Period  (1988-1990)

 

                      I started  my research work in 1988 with the study on the antitumer activity of methylglyoxal . This study showed that methylglyoxal specifically inhibits the respiration of malignant cells without inhibiting the respiration of normal cells. Simultaneously I have purified and characterized an enzyme named as aminoacetone synthase from goat liver mitochondria [Biochemical . J. (1991) 275, 575-579].

 

Doctoral Period  (1990-1994)

 

      In 1990, when I was selected for National Fellowship towards the Ph.D. degree, I decided to do my Ph.D. work with a protozoan pathogen L.donovani that is the causative agent for kala-azar, a serious health hazard in many parts of the tropical world, including India. It was my desire to work on some problem of national interest. This protozoan pathogen has a digenic life cycle. The flagellated promastigote or vector form transforms in liver macrophages to flagellated pathogenic amastigote.

               In my Ph.D. work, I have shown that Leishmania donovani promastigotes do not require extracellular Ca2+ for its growth and division but amastigote to promastigote transformation is strictly dependent on extracellular Ca2+. Disruption of Ca2+ homeostasis also affects the transformation process [Prop. Indian National Sci. Acad.  B58 No.5, pp 281-288 (1992). With the help of permeabilization technique, I have shown that along with endoplasmic reticulum and mitochondria Leishmania sp. possess a third organelle as major intracellular Ca2+ pool (unpublished). I have also tried to investigate the energy metabolism in permeabilized Leishmania donovani promastigote cells. With the help of fluorimetric techniques I have shown that temperature shift acts as a signal to elevate cytoplasmic free Ca2+. Accumulated results, both on promastigote and amastigote stage in different pathogenic and nonpathogenic strains indicates the possibility of the involvement of temperature sensitive Ca2+ pool in the pathogenicity of the organism [FEBS LETTS 375, pp83-86 (1995)].

      In AstraZeneca (1996-2002)  

                   Most of the period I spent here developing assays for primary and secondary screens in HTS programs.  I was also involved in elucidating the catalytic pathways and mechanism of action of the enzyme Hypoxanthine-Guanine-Xanthine phosphoribosyl transferase from Plasmodium falciparam. From kinetic studies it is shown that this enzyme follows a Ping-Pong type mechanism. I have also shown by chemical modification and site directed mutagenesis that a critical histidine residue is involved in xanthine selectivity of the enzyme [Mol. Biochem. Parasitol (2004) 137(2) pp 267-276].  After that I was assigned to different projects with different kind of responsibilities. In brief, my major achievements are (1) development of an HTS assay (bacterial RNA polymerase) and its transfer to HTS facility at Aldreley Park UK, where screening of 1million compounds was carried out, (2) fluorescent substrate preparation for whole HTS campaign (Bhat et. al. 2006), (6) setting up of a coupled assay of MraY and MurG (E.coli) for secondary screening, (3) purification of two proteins and a plasmid in large scale for secondary screening, (4) setting up of bacterial respiration studies using oxygraph instrument for secondary screening, and (5) membrane preparation for another HTS assay (bacterial peptidoglycan biosynthesis) required for screening campaign of 1million compounds, (6)  setting up of a coupled assay of MraY and MurG (E.coli) for secondary screening, etc.

 

In  NCL ( 2002- present)

                  Since my joining at National Chemical Laboratory, CSIR, Pune on 4th March, 2002, apart from taking the responsibility of setting up of High Throughput Screening Facility, I started basic research on analyzing the importance of nitrogen metabolic pathway for identifying novel compounds as inhibitor of tuberculosis bacilli (Khan et. al. 2008, Khan et.al 2008). Controlling tuberculosis is one of the major concerns India is trying to solve. We have already developed few target based and whole cell based high throughput screening protocols for identifying novel inhibitors (Singh et al. 2005; Khan et.al 2008; Singh and Sarkar 2006; Singh et. al. 2010). Availing the advantage of being in NCL, my group paid attention in screening different libraries in collaboration with the synthetic organic chemists and already identified few novel anti-tubercular scaffolds (Complete specification filed at PCT, 2011; Provisional Patents filed in 2010). Our results clearly showed that there is an active nitrate/ammonia metabolic pathway present in the mycobacterium bacilli that helps in survival of the pathogen in host (Akhtar et. Al. 2013). Recently our studies on 1,2,4-trialzolethiols has clearly established that chaperonin 2 as an anti-tubercular target (Provisional Patent filed 2011). As part of NRM activity, I have already developed a screening facility with a library of 3000 natural products extracts/fractions as well as 3000 synthetic compounds. Biochemical characterization has revealed a mechanism based enzyme inactivation occurs as a result of interaction between glutamine synthetase and its inhibitor L-methionine S-sulfoximine (Unpublished observation). A detailed study from our group on host-pathogen interaction has revealed the development of hypoxic environment within infected macrophages when glutamine synthetase as well as nitrate reductase plays important role in the survival of the bacilli before necrosis of the host cell to start another round of infection (Provisional Patent 2011).

                          Along with nitrogen metabolic pathway, we are also involved in identifying the role of cellular redox components in development and maintenance of latent stage of the bacilli. Our unpublished observation suggests that hydroxyl radical as a signal for activation of tubercular bacilli from its latent stage. Currently, we are involved in identifying the proteins involved in this signaling cascade leading to activation of the pathogen which is an important physiological event for sudden development of disease in human. It has also been observed that Identified scaffolds from our facility are being tested for their in vivo studies on different models. I have also standardized a) Antiproliferation assay using MTT dye, b) Antiapoptotic assay using Caspase 3/7 kit and c) Cytotoxicity assay using LDH release detection kit on 12 different cell lines as part of my plan to enter into anti-cancer development program. Apart from screening programs, we are currently involved in biochemical evaluation of potential targets, their interaction with the known inhibitors and identification of targets for our lead molecules by applying proteomic approach as well as understanding the molecular basis of reactivation of dormant bacilli into the active stage as part of our research interest.

 

 

Selected Publications

  • Yeware AM, Shurpali KD And Athalye MC and Sarkar D , Superoxide Generation and Its Involvement in the Growth of Mycobacterium smegmatis, Front. Microbiol., 8:105, (2017), DOI:10.3389/fmicb.2017.00105.
  • Sambhaji T. Dhumal · Amarsinh R. Deshmukh · Manisha R. Bhosle · Vijay M. Khedkar · Laxman U. Nawale · Dhiman Sarkar · Ramrao A. Mane, Synthesis and Antitubercular Activity of New 1,3,4-Oxadiazoles Bearing Pyridyl and Thiazolyl Scaffolds, Bioorganic & Medicinal Chemistry Letters., , (2016), DOI:http://dx.doi.org/10.1016/j.bmcl.2016.05.093.
  • Singh R, Nawale L, Arkile M, Wadhwani S, Shedbalkar U, Chopade S, Sarkar D And Chopade BA, Phytogenic silver, gold, and bimetallic nanoparticles as novel antitubercular agents, International Journal of Nanomedicine., Volume 11, 1889 - 1897 (2016), DOI:https://dx.doi.org/10.2147/IJN.S102488.
  • Honmore VS1, Kandhare AD2, Kadam PP2, Khedkar VM3, Sarkar D3, Bodhankar SL4, Zanwar AA5, Rojatkar SR6 And Natu AD7, Isolates of Alpinia officinarum Hance as COX-2 inhibitors: Evidence from anti-inflammatory, antioxidant and molecular docking studies, International Immunopharmacology., 33, 8 - 17 (2016), DOI:doi:10.1016/j.intimp.2016.01.024.
  • Chitre TS1, Asgaonkar KD2, Miniyar PB3, Dharme AB2, Arkile MA4, Yeware A4, Sarkar D4, Khedkar VM5 And Jha PC6, Synthesis and docking studies of pyrazine-thiazolidinone hybrid scaffold targeting dormant tuberculosis, Bioorganic & Medicinal Chemistry Letters., 26, 2224 - 2228 (2016), DOI:doi:10.1016/j.bmcl.2016.03.055.
  • Ashish A. Chinchansure1, Manisha Arkile2, Dinesh R. Shinde3, Dhiman Sarkar2 And Swati P. Joshi1, A New Dinor-cis-Labdane Diterpene and Flavonoids with Antimycobacterium Activity from Colebrookea oppositifolia, Planta Medica Letters., , (2016), DOI:10.1055/s-0042-102200.
  • Dnyaneshwar D. Subhedara, Mubarak H. Shaikha, Laxman Nawaleb, Amar Yewareb, Dhiman Sarkarb, Firoz A. Kalam Khanc, Jaiprakash N. Sangshettic, Bapurao B. Shingatea, And , Novel Tetrazoloquinoline-Rhodanine Conjugates: Highly Efficient Synthesis and Biological Evaluation, Bioorganic & Medicinal Chemistry Letters., 26, 2278 - 2283 (2016), DOI:doi:10.1016/j.bmcl.2016.03.045.
  • Dnyaneshwar D. Subhedar · Mubarak H. Shaikh · Laxman Nawale · Amar Yeware · Dhiman Sarkar · Bapurao B. Shingate, [Et3NH][HSO4] catalyzed efficient synthesis of 5-arylidene-rhodanine conjugates and their antitubercular activity, Research on Chemical Intermediates., , (2016), DOI:10.1007/s11164-016-2484-0.
  • Varsha S. Honmorea, Supada R. Rojatkarb, Laxman U. Nawalec, Manisha A. Arkilec, Vijay M. Khedkarc, Arun D. Natua And Dhiman Sarkarc, In vitro and ex vivo antitubercular activity of diarylheptanoids from the rhizomes of Alpinia officinarum Hance, Natural Product Research., , (2016), DOI:10.1080/14786419.2016.1149702.
  • Priyanka R. Sharma · Sunil Kamble · Dhiman Sarkar · Amitesh Anand · Anjani J. Varma, Shape and size engineered cellulosic nanomaterials as broad spectrum anti-microbial compounds, nternational Journal of Biological Macromolecules., 87, 460 - 465 (2016), DOI:10.1016/j.ijbiomac.2016.02.024.
  • N.C. Desai · Hardik Somani · Amit Trivedi · Kandarp Bhatt · Laxman Nawale · Vijay M. Khedkar · Prakash C. Jha · Dhiman Sarkar, Synthesis, biological evaluation and mo lecular docking study of some novel indole and pyridine based 1,3,4-oxadiaz ole derivatives as potential antituber- cular agents, Bioorganic & Medicinal Chemistry Letters., 26, 1776 - 1783 (2016), DOI:10.1016/j.bmcl.2016.02.043.
  • Dnyaneshwar D. Subhedar · Mubarak H. Shaikh · Manisha A. Arkile · Amar Yeware · Dhiman Sarkar · Bapurao B. Shingate, Facile Synthesis of 1,3-Thiazolidin-4-ones as Antitubercular Agents, Bioorganic & Medicinal Chemistry Letters., 26, 1704 - 1708 (2016), DOI:10.1016/j.bmcl.2016.02.056.
  • Ruby Singh , Laxman Nawale , Dhiman Sarkar , C. G. Suresh (Contributed equally to this work: Ruby Singh And Laxman Nawale), Two Chitotriose-Specific Lectins Show Anti- Angiogenesis, Induces Caspase-9-Mediated Apoptosis and Early Arrest of Pancreatic Tumor Cell Cycle, PLoS ONE., , (2016), DOI:http://dx.doi.org/10.1371/journal.pone.0146110.
  • Tabasum Ismail · Syed Shafi · Jada Srinivas · Dhiman Sarkar · Yasrib Qurishi · Jabeena Khazir · Mohammad Sarwar Alam · Halmuthur Mahabalarao Sampath Kumar, Synthesis and Tyrosinase Inhibition activity of trans-Stilbene Derivatives, Bioorganic Chemistry., 64, 97 - 102 (2016), DOI:10.1016/j.bioorg.2016.01.001.
  • Mubarak H. Shaikh, Bapurao B. Shingat, Laxman Nawale, Govinda R. Navale And Sandip S. Shinde, Synthesis, biological evaluation and molecular docking of novel coumarin incorporated triazoles as antitubercular, antioxidant and antimicrobial agents, Medicinal Chemistry Research., 25, 790 - 804 (2016), DOI:10.1007/s00044-016-1519-9.
  • Mubarak H. Shaikh · Dnyaneshwar D. Subhedar · Firoz A. Kalam Khan · Jaiprakash N. Sangshetti · Laxman Nawale · Manisha Arkile · Dhiman Sarkar · Bapurao B. Shingate, Synthesis of Novel Triazole-incorporated Isatin Derivatives as Antifungal, Antitubercular, and Antioxidant Agents and Molecular Docking Study, Journal of Heterocyclic Chemistry., 9999, (2016), DOI:10.1002/jhet.2598.
  • Mubarak H. Shaikh · Dnyaneshwar D. Subhedar · Manisha Arkile · Vijay M. Khedkar · Nandadeep Jadhav · Dhiman Sarkar · Bapurao B. Shingate, Synthesis and bioactivity of novel triazole incorporated benzothiazinone derivatives as antitubercular and antioxidant agent, Bioorganic & Medicinal Chemistry Letters., 26, 561 - 569 (2016), DOI:doi:10.1016/j.bmcl.2015.11.071.
  • Pravin S. Mahajan†, Mukesh D. Nikam†, Laxman U. Nawale‡, Vijay M. Khedkar‡, Dhiman Sarkar‡ And and Charansingh H. Gill*†, Synthesis and Anti-tubercular Activity of New Benzo[b]thiophenes, ACS Med. Chem. Lett., , (2016), DOI:10.1021/acsmedchemlett.6b00077.
  • Pradip K. Gadekar, Abhijit Roychowdhury, Prashant S. Kharkar, Vijay Khedkar, Manisha Arkile, Hardik Manek, Dhiman Sarkar, Rajiv Sharma, V. Vijayakumar And S. Sarveswari, Design, synthesis and biological evaluation of novel azaspiro analogs of linezolid as antibacterial and antitubercular agents, European Journal of Medicinal Chemistry., , (2016), DOI:10.1016/j.ejmech.2016.07.001.
  • Dnyaneshwar D. Subhedar, Mubarak H. Shaikh, Bapurao B. Shingate, Laxman Nawale And Dhiman Sarkar and Vijay M. Khedkar, Novel tetrazoloquinoline–thiazolidinone conjugates as possible antitubercular agents: synthesis and molecular docking, Med. Chem. Commun., , (2016), DOI:10.1039/C6MD00278A.
  • Pal S, Ramu V, Taye N, Mogare DG, Yeware AM, Sarkar D, Reddy DS, Chattopadhyay And Das A, GSH Induced Controlled Release of Levofloxacin form a Pur-pose-built Prodrug: Luminescence Response for Probing the Drug Release in E-coli and Staphylococcus aureus, Bioconjugate Chemistry., , (2016), DOI:10.1021/acs.bioconjchem.6b00324.
  • Dnyaneshwar D. Subhedar, Mubarak H. Shaikh, Bapurao B. Shingate, Laxman Nawale, Dhiman Sarkar, Vijay M. Khedkar, Firoz A. Kalam Khan And Jaiprakash N. Sangshetti, Quinolidene-rhodanine conjugates: Facile synthesis and biological evaluation, European Journal of Medicinal Chemistry., , (2016), DOI:10.1016/j.ejmech.2016.09.059.
  • Jaisingh M. Divse, Santosh B. Mhaske, Chitanya R. Charolkar, Duhita G Sant, Santosh G Tupe, Mukund V. Deshpande, vijay khedkar, Laxman Nawale And Dhiman Sarkar and Vandana Sudhir Pore, Synthesis and biological evaluation of new fluconazole ?-lactam conjugates linked via 1,2,3-triazole, New Journal of Chemistry., , (2016), DOI:10.1039/C6NJ03117J.
  • Mahendra B. Bhalerao, Sambhaji T. Dhumal, Amarsinh R. Deshmukh, Laxman U. Nawale, Vijay Khedkar, Dhiman Sarkar And Ramrao A. Mane, New bithiazolyl hydrazones: novel synthesis, characterization and antitubercular evaluation, Bioorganic & Medicinal Chemistry Letters., , (2016), DOI:http://dx.doi.org/10.1016/j.bmcl.2016.11.056.
  • Tushar R. Valkute · Manisha Arkile · Dhiman Sarkar · Asish K. Bhattacharya, Naturally Occurring Anti-TB Agents: Isolation, Chemical Transformations and In Vitro Antitubercular Activities of Secondary Metabolites of Rhizomes of Alpinia galanga, Planta Medica., , (2016), DOI:10.1055/s-0042-112226.
  • Hanumant Bapurao Borate · Ritesh A. Annadate · Sandip S. Vagh · Mahesh M. Pisal · Sagar B. Deokate · Manisha A. Arkile · Nandadeep J. Jadhav · Laxman U. Nawale · Dhiman Sarkar, Synthesis and evaluation of thieno[2,3-d]pyrimidin-4(3H)-ones as potential antitubercular agents, Medicinal Chemistry Communication., , (2015), DOI:10.1039/C5MD90062J.
  • Arundhati C. Lele · Archana Raju · Mihir P. Khambete · M. K. Ray · M. G. R. Rajan · Manisha A. Arkile · Nandadeep J. Jadhav · Dhiman Sarkar · Mariam S. Degan, Design and Synthesis of a Focused Library of Diamino Triazines as Potential Mycobacterium tuberculosis DHFR Inhibitors, ACS Med Chem Lett., 6 (11), 1140 - 1144 (2015), DOI:10.1021/acsmedchemlett.5b00367.
  • Vandana S. Pore, Jaisingh M. Divse, Chaitanya R. Charolkar, Laxman U. Nawale, Vijay M. Khedkar And Dhiman Sarkar, Design and synthesis of 11? - substituted bile acid derivatives as potential anti - tuberculosis agents, Bioorganic & Medicinal Chemistry Letters., 25, 4185 - 4190 (2015), DOI:10.1016/j.bmcl.2015.08.006.
  • Ashish Chinchansure · Neelam Shamnani · Manisha Arkile · Dhiman Sarkar · Swati Joshi, ANTIMYCOBACTERIUM ACTIVITY OF COUMARINS FROM FRUIT PULP OF AEGLE MARMELOS (L.) CORREA, International Journal of Basic and Applied Chemical Sciences., 5, 39 - 44 (2015).
  • Pravin S. Mahajan · Mukesh D. Nikam · Vijay M. Khedkar · Prakash C. Jha · Dhiman Sarkar · Charansingh H. Gill, Synthesis, biological evaluation and molecular docking studies of N-acylheteroaryl hydrazone derivatives as antioxidant and anti-inflammatory agents, Research on Chemical Intermediates., , (2015), DOI:10.1007/s11164-015-2176-1.
  • Sarvesh K Soni · Sampa Sarkar · Selvakannan R. Periasamy · Dhiman Sarkar · Suresh K Bhargava, Intrinsic therapeutic and biocatalytic roles of ionic liquid mediated self-assembled platinum-phytase nanosphere, RSC Advances., 5, 62871 - 62881 (2015), DOI:10.1039/c5ra11273g.
  • Ashish Chinchansure · Manisha Arkile · Anurag Shukla · Shanmugam Dhanasekaran · Dhiman Sarkar · Swati P. Joshi, Leucas mollissima, a Source of Bioactive Compounds with Antimalarial and Antimycobacterium Activities, Planta Medica Letters., 2, 35 - 38 (2015), DOI:10.1055/s-0035-1557830.
  • Rima Bharadwaj · Ashish A. Chinchansure · Roshan R. Kulkarni · Manisha Arkile · Dhiman Sarkar · Swati P. Joshi, Rottlerin Derivatives and Other Compounds from Mallotus philippinensis Fruits and Their Potential Antimycobactrial Activity, Planta Medica Letters., 2, 28 - 30 (2015), DOI:10.1055/s-0035-1557793.
  • Nilesh R Tawari · Seema Bag · Archana Raju · Arundhati C Lele · Ranjeet Bairwa · Mukti Kanta Ray · Mgr Rajan · Laxman U Nawale · Dhiman Sarkar · Mariam S Degani, Rational drug design, synthesis and biological evaluation of dihydrofolate reductase inhibitors as antituberculosis agents, Future medicinal chemistry., 7(8), 979 - 88 (2015), DOI:10.4155/fmc.15.48.
  • Roshan R Kulkarni · Ketaki Shurpali · Vijay M Khedkar · Vedavati G Puranik · Dhiman Sarkar · Swati P Joshi, New pimarane diterpenes and other antimycobacterial metabolites from Anisochilus verticillatus, Natural product research., 30(6), 675 - 681 (2015), DOI:10.1080/14786419.2015.1040990.
  • Mubarak H. Shaikh · Dnyaneshwar D. Subhedar · Laxman Nawale · Dhiman Sarkar · Firoz A. Kalam Khan · Jaiprakash N. Sangshetti · Bapurao B. Shingate, 1,2,3-Triazole derivatives as antitubercular agents: synthesis, biological evaluation and molecular docking study, Medicinal Chemistry Communication., 6, 1104 - 1116 (2015), DOI:10.1039/c5md00057b.
  • Richa Singh · Laxman U Nawale · Manisha Arkile · Utkarsha U Shedbalkar · Sweety A Wadhwani · Dhiman Sarkar · Balu A Chopade, Chemical and biological metal nanoparticles as antimycobacterial agents: A comparative study, International Journal of Antimicrobial Agents., 46(2), 183 - 8 (2015), DOI:10.1016/j.ijantimicag.2015.03.014.
  • Abhishek Mishra · Dhiman Sarkar, Qualitative and quantitative proteomic analysis of Vitamin C induced changes in Mycobacterium smegmatis, Frontiers in Microbiology., 6, 451 (2015), DOI:10.3389/fmicb.2015.00451.
  • Parul Dubey · Laxman Nawale · Dhiman Sarkar · Anuya Nisal · Asmita A Prabhune, Sophorolipid assisted tunable and rapid gelation of silk fibroin to form porous biomedical scaffolds, RSC Advances., 5, 33955 - 33962 (2015), DOI:10.1039/C5RA04317D.
  • Madhukar S Said · Ashish A Chinchansure · Laxman Nawale · Ankita Durge · Ashish Wadhwani · Smita S Kulkarni · Dhiman Sarkar · Swati · P Joshi, A new butenolide cinnamate and other biological active chemical constituents from Polygonum glabrum, Natural product research., 29(22), 2080 - 6 (2015), DOI:10.1080/14786419.2015.1004674.
  • Sambhaji T. Dhumal · Amarsinh R. Deshmukh · Lalit D. Khillare · Manisha Arkile · Dhiman Sarkar · and Ramrao A. Mane, Synthesis and Antitubercular activity of New Thiazolidinones with Pyrazinyl and Thiazolyl scaffolds, Journal of Heterocyclic Chemistry., , (2015), DOI:10.1002/jhet.2552.
  • Shamim Akhtar · Sheetal Singh · Santosh Kumar · Dhiman Sarkar, Analyzing Different Acetyl Co-A Metabolizing Enzymes as Potential Drug Targets against Mycobacterium tuberculosis, British Microbiology Research Journal., 10, (2015), DOI:10.9734/BMRJ/2015/19032.
  • Preeti Nigam · Dhiman Sarkar, Multifunctional Silica Nanoparticles for Pancreatic Cancer Specific Drug Delivery and Bioimaging, Journal ofChemistry & Applied Biochemistry., 2, (2015).
  • Laxman Nawale · Hardik Manek · Nandadeep Jadhav · Jennifer Cheruvathur · Dhiman Sarkar, High Throughput Screening of Inhibitors for Tuberculosis at NCL : A Novel Drug Discovery Initiative from CSIR, Conference Paper., , (2015).
  • Ruby Singha · Laxman Nawaleb · Sushama M. Gaikwada · Dhiman Sarkarb · C.G. Suresha, Purification and characterization of an agglutinin from Datura innoxia with antineoplastic activity, Conference Paper., , (2015).
  • Parul Dubeya · Laxman Nawaleb · Dhiman Sarkarb · Anuya Nisalc · Asmita Prabhunea, Mechanistic understanding of rapid gelation of Silk fibroin using a biosurfactant – Sophorolipid, Conference Paper., , (2015).
  • Vivek Khilari · Laxman Nawale · Dhiman Sarkar · Bhaskar Idage · Susheela Idage, BIODRGRADABLE CHITOSAN GRAFT POLY(LACTIC ACID) NANOPARTICLES AS NANOCARRIERS FOR ANTI-TB DRUG (ISONIAZID), Conference Paper., , (2015).
  • Laxman Nawale · Dhiman Sarkar, Discovery of new chemical entities against diseases of national importance :an HTS approach, Conference Paper., , (2015).
  • Laxman Nawale · Dhiman Sarkar, A QUANTITATIVE, AUTOMATED, HIGH CONTENT SCREENING ASSAY APPROACH TO ANTI-ANGIOGENICDRUG DISCOVERY, Conference Paper., , (2015).
  • Laxman Nawale · Dhiman Sarkar, Efficacy Study Of Potential Anti-tubercular Molecules: In-vitro and Ex-vivo, Conference Paper., , (2015).
  • Roshan R. Kulkarni, Santosh G. Tupe, Suwarna P. Gample, Macchindra G. Chandgudea, Dhiman Sarkar And Mukund V. Deshpande & Swati P. Joshi, Antifungal dimeric chalcone derivative kamalachalcone E from Mallotus philippinensis, Natural Product Research., 28, 245 - 250 (2014), DOI:10.1080/14786419.2013.843178.
  • Preeti Nigam · Shobha Waghmode · Amar Yeware · Laxman Nawale · Priyanka Dagde · Amol Dhudhane · Dhiman Sarkar, Aptamer Functionalized Multifunctional Fluorescent Nanotheranostic Platform for Pancreatic Cancer, Journal of Nanopharmaceutics and Drug Delivery., 2, 280 - 287 (2014), DOI:10.1166/jnd.2014.1070.
  • Ketaki Dilip Shurpali · Upasana Singh · Shamim Akhtar Sampa Sarkar · Abhishek Mishra · Roshan Kulkarni · Swati Joshi · Dhiman Sarkar, Advanced cell-based high-throughput screening assays targeting active and latent Mycobacterium tuberculosis, Journal of Biotechnology., 185, S18 (2014), DOI:10.1016/j.jbiotec.2014.07.061.
  • Rohit R Joshi · Avinash Barchha · Vijay M Khedkar · Raghuvir R S Pissurlenkar · Sampa Sarkar · Dhiman Sarkar · Rohini R Joshi · Ramesh A Joshi · Anamik K Shah · Evans C Coutinho, Targeting Dormant Tuberculosis Bacilli: Results for Molecules with a Novel Pyrimidone Scaffold, Chemical Biology & Drug Design., 85(2), 201 - 207 (2014), DOI:10.1111/cbdd.12373.
  • Preeti Nigam · Shobha Waghmode · Michelle Louis · Shishanka Wangnoo · Pooja Chavan · Dhiman Sarkar, Materials Chemistry B, Journal of Materials Chemistry., , (2014), DOI:10.1039/c4tb00015c.
  • Shamim Akhtar, Arshad Khan, Charles D. Sohaskey And Chinnaswamy Jagannath and Dhiman Sarkar, Nitrite Reductase NirBD is induced and plays and important role during in vivo dormancy of Mycobacterium tuberculosis, Journal of Bacteriology., vol. 195 no. 20, 4592 - 4599 (2013).
  • Roshan R. Kulkarni, Ketaki Shurpali, Vedavati G. Puranik And Dhiman Sarkar and Swati P. Joshi, Antimycobacterial Labdane Diterpenes from Leucas stelligera, Journal of Natural Products., 76 (10), 1836 - 1841 (2013).
  • Rohan A. Limaye, Virendra B. Kumbhar, Arun D. Natu, Madhusudan V. Paradkar, Varsha S. Honmore, Rubia R. Chauhan, Suwarna P. Gample And Dhiman Sarkar, One pot solvent free synthesis and in vitro antitubercular screening of 3-Aracylphthalides against Mycobacterium tuberculosis, Bioorganic & Medicinal Chemistry Letters., 23, 711 - 714 (2013), DOI:10.1016/j.bmcl.2012.11.097.
  • Shafeek A. R. Mulla · Mohsinkhan Y. Pathan · Santosh S. Chavan · Suwara P. Gample · Dhiman Sarkar, Highly efficient one-pot multi-component synthesis of a-aminophosphonates and bis-a- aminophosphonates catalyzed by heterogeneous reusable silica supported dodecatungstophosphoric acid (DTP/SiO2) at ambient temperature and their antitubercular evaluation against Mycobactrium Tuberculosis, RSC Advances., 4, 7666 - 7672 (2013), DOI:10.1039/C3RA45853A.
  • Roshan R Kulkarni · Santosh G Tupe · Suwarna P Gample · Macchindra G Chandgude · Dhiman Sarkar · Mukund V Deshpande · Swati P Joshi, Antifungal dimeric chalcone derivative kamalachalcone E from Mallotus philippinensis, Natural product research., 28, 245 - 250 (2013), DOI:10.1080/14786419.2013.843178.
  • Roshan R Kulkarni · Ketaki Shurpali · Vedavati G Puranik · Dhiman Sarkar · Swati P Joshi, Antimycobacterial Labdane Diterpenes from Leucas stelligera, J Nat Prod., 76, 1836 - 1841 (2013), DOI:10.1021/np400002p.
  • Shamim Akhtar · Arshad Khan · Charles D Sohaskey · Chinnaswamy Jagannath · Dhiman Sarkar, Nitrite Reductase NirBD Is Induced and Plays an Important Role during In Vitro Dormancy of Mycobacterium tuberculosis, Journal of bacteriology., 195, 4592 - 4599 (2013), DOI:10.1128/JB.00698-13.
  • Sampa Sarkar And Dhiman Sarkar, Potential use of nitrate reductase as a biomarker for the identification of active and dormant inhibitors of Mycobacterium tuberculosis in a THP-1 infection model, Journal of Biomolecular Screening., vol. 17 no. 7, 966 - 973 (2012).
  • Rohan A Limaye · Virendra B Kumbhar · Arun D Natu · Madhusudan V Paradkar · Varsha S Honmore · Rubia R Chauhan · Suwarna P Gample · Dhiman Sarkar, ChemInform Abstract: One Pot Solvent Free Synthesis and in vitro Antitubercular Screening of 3-Aracylphthalides Against Mycobacterium tuberculosis, Bioorganic & Medicinal Chemistry Letters., , (2012), DOI:10.1002/chin.201321107.
  • Roshan R Kulkarni · Ketaki Shurpali · Rupesh L Gawde · Dhiman Sarkar · Vedavati G Puranik · Swati P Joshi, Phyllocladane diterpenes from Anisomeles heyneana, Journal of Asian natural products research., , (2012).
  • R. R. Kulkarni · K. D. Shurpali · R. I. Gawde · D. Sarkar · V. G. Puranik · S. P. Joshi, Chemical investigation of Plectranthus mollis, Journal of Medicinal and Aromatic Plant Sciences., , (2012).
  • Shamim Akhtar, Sampa Sarkar, Abhishek Mishra And Dhiman Sarkar, A method to extract intact and pure RNA from mycobacteria, Analytical Biochemistry., 417(2), 286 - 288 (2011).
  • Sarvesh K Soni · Sampa Sarkar · Dhiman Sarkar · Peter J Coloe · Suresh K Bhargava · Vipul Bansal, SELF–ASSEMBLED HISTIDINE ACID PHOSPHATE NANOCAPSULES AS TEMPLATES FOR MULTIFUNCTIONAL HOLLOW PLATINUM NANOSPHERES IN IONIC LIQUID [BMIM][BF 4 ] FOR DRUG DELIVERY, Proceedings of Chemeca., , (2011).
  • Arshad Khan · Dhiman Sarkar, Nitrate reduction pathways in mycobacteria and their implications during latency, Microbiology., 158, 301 - 307 (2011), DOI:10.1099/mic.0.054759-0.
  • Upasana Singh · Shamim Akhtar · Abhishek Mishra · Dhiman Sarkar, A novel screening method based on menadione mediated rapid reduction of tetrazolium salt for testing of anti-mycobacterial agents, Journal of microbiological methods., 84, 202 - 207 (2011), DOI:10.1016/j.mimet.2010.11.013.
  • A. Khan And D. Sarkar, A simple whole cell based high throughput screening protocol using Mycobacterium bovis BCG for inhibitors against dormant and active tubercle bacilli, Microbiol Methods., 73(1), 62 - 68 (2008).
  • A. Khan, S. Sarkar And D. Sarkar, Bactericidal activity of 2-nitroimidazole against the active replicating stage of Mycobacterium bovis BCG and Mycobacterium tuberculosis with intracellular efficacy in THP-1 macrophages, Int. J Antimicrob Agents., 32(1), 40 - 45 (2008).
  • Arshad Khan · Shamim Akhtar · Jawid N Ahmad · Dhiman Sarkar, Presence of a functional nitrate assimilation pathway in Mycobacterium smegmatis, Microbial Pathogenesis., 44, 71 - 77 (2008).
  • J Bhat, R Rane, S M Solapure, D Sarkar, U Sharma, M N Harish, S Lamb, D Plant, P Alcock, S Peters, S Barde And R K Roy, High-throughput screening of RNA polymerase inhibitors using a fluorescent UTP analog, J. Biomol. Screen., 11(8), 968 - 976 (2006).
  • U Singh And D Sarkar, Development of a simple high-throughput screening protocol based on biosynthetic activity of Mycobacterium tuberculosis glutamine synthetase for the identification of novel inhibitors, J Biomolecular Screening., 11(8), 1035 - 1042 (2006).
  • A Khan And D Sarkar, Identification of a respiratory-type nitrate reductase and its role for survival of Mycobacterium smegmatis in Wayne model, Microb Pathog., 41(2-3), 90 - 95 (2006).
  • Upasana Singh · Vinita Panchanadikar · Dhiman Sarkar, Development of a Simple Assay Protocol for High-Throughput Screening of Mycobacterium tuberculosis Glutamine Synthetase for the Identification of Novel Inhibitors, Journal of Biomolecular Screening., 10, 725 - 729 (2005).
  • D Sarkar, I Ghosh And S Datta, Biochemical characterization of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphorbosyltransferase: role of histidine residue in substrate selectivity, Mol Biochem Parasitol., 137(2), 267 - 276 (2004).
  • D. Sarkar and A. Bhaduri, Temperature induced rapid rise in cytoplasmic free Ca2+ concentration in pathogenic Leishmania donovani promastigotes, FEBS letters., 375, 83 - 86 (1995).
  • Dhiman Sarkar · Anil Ghosh · Amarnath Bhaduri, Possible involvement of Ca2+ in the in vitro morphogenetic transformation of Leishmania donovani amastigotes to promastigotes, Proceedings of the Indian National Science Academy. Part B Biological sciences., 58, 281 - 288 (1992).
  • M. Ray, D. Sarkar And S. Ray, Aminoacetone synthase from goat liver involvement of arginine residue at the active site and on the stability of the enzyme, J Biochem., 275, 575 - 579 (1991).

Research Interest

  • Enzymology AND Microbiology

Contact Details

Combi Chem-Bio Resource Centre,

Division of Organic Chemistry,

CSIR-National Chemical Laboratory,

Dr. Homi Bhabha Road,

Pune - 411008

India