H. V. Thulasiram

H. V. Thulasiram

Organic Chemistry Division

About Me

We work in the area of isoprenoid biosynthetic pathways, biocatalysis and assay based isolation and characterization of natural products.

The isoprenoid biosynthetic pathways provide intermediates for the synthesis of a vast variety of structurally and chemically diverse natural products that serve numerous biochemical functions in living systems. We are interested in elucidation of the isoprenoid biosynthetic pathways with special emphasis on establishing the mechanisms of the enzyme-catalyzed transformations and to understand, how the enzymes promote the reactions using the concepts and tools of molecular biology, biochemistry, synthetic biology, and organic chemistry. 

Our group also involved in utilization of nature's catalysts in organic synthesis. We screen the whole cell microorganisms or isolated enzymes, which can carryout the desired transformation on natural products and xenobiotics which are of biological and commercial interest.

Our lab is well equipped and established for conducting the research in multidisciplinary area of Chemical Biology.

Professional Experience

Scientist Gr IV (4) [ Pr. Scientist); 14/08/10 – Present; National Chemical Laboratory, Pune.

Scientist Gr IV (3) [Sr. Scientist); 14/08/06 – 14/08/10; National Chemical Laboratory, Pune.

Selected Publications

  • Pandreka A, Dandekar DS, Haldar S, Uttara V, Vijayashree SG, Mulani FA, Aarthy T And Thulasiram HV. , Triterpenoid Profiling and Functional Characterization of the Initial Genes Involved in Isoprenoid Biosynthesis in Neem (Azadirachta indica), BMC Plant Biology., 15:214, 1 - 14 (2015), DOI:10.1186/s12870-015-0593-3.
  • Krithika R, Srivastava PL, Rani B, Kolet SP, Chopade M, Mantri S And Thulasiram HV, Characterization of 10-Hydroxygeraniol Dehydrogenase from Catharanthus roseus Reveals Cascaded Enzymatic Activity in Iridoid Biosynthesis, Scientific Reports (Nature Group)., 5, 8258 (2015), DOI:10.1038/srep08258.
  • Srivastava PL, Daramwar PP, Krithika R, Pandreka A, Shankar SS And Thulasiram HV, Functional Characterization of Novel Sesquiterpene Synthases from Indian Sandalwood, Santalum album, Scientific Reports (Nature Group)., 5, 10095 (2015), DOI:10.1038/srep10095.
  • Haldar S, Mulani FA, Aarthy T And Thulasiram HV, 11?-Hydroxylation on the Basic Limonoid Skeleton by Fungal Biocatalyst, Cunninghamella echinulata, J. Org. Chem., 80, 6490 - 6495 (2015), DOI:10.1021/acs.joc.5b00417.
  • Jadhav S, Paphale PB, Thulasiram HV And Bhargava S, Polyketide synthesis in tobacco plants transformed with Plumbago zeylanica hexaketide synthase, Phytochemistry., 98, 92 - 100 (2014).
  • Daramwar PP, Srivastava PL, Kolet SP And Thulasiram HV. , Biocatalyst mediated regio- and stereo-selective hydroxylation and epoxidation of (Z)-?-Santalol, Org. Biomol. Chem., 12, 1048 - 1051 (2014).
  • Haldar S, Kale B, Jadhav DD And Thulasiram HV, Lipase mediated separation of triterpene structural isomers, ?- and ?-amyrin, Tetrahedron Lett., 55, 3122 - 3125 (2014).
  • Kolet SP, Jadhav DD, Priyadarshini B, Swarge BN And Thulasiram HV, Fungi mediated production and practical purification of (R)-(-)-3-quinuclidinol, Tetrahedron Lett., 55, 5911 - 5914 (2014).
  • Shankar S, Benke SN, Nagendra N, Srivastava PL, Thulasiram HV And Gopi HN, Self-Assembly to Function: Design, Synthesis and Broad Spectrum Antimicrobial Properties of Short Hybrid E-Vinylogous Lipopeptides, J. Med. Chem., 56, 8468 - 8474 (2013).
  • Haldar S, Kumar S, Kolet SP, Patil HS, Kumar D, Kundu G And Thulasiram HV, One-Pot Fluorescent Labeling Protocol for Complex Hydroxylated Bioactive Natural Products, J. Org. Chem., 78, 10192 - 10202 (2013).
  • Kolet SP, Niloferjahan S, HaldarS, Gonnade R And Thulasiram HV. , Biocatalyst Mediated Production of 6 beta,11 alpha-dihydroxy derivatives of 4-ene-3-one Steroids, Steroids., 78, 1152 - 1158 (2013).
  • Haldar S, Phapale PB, Kolet SP And Thulasiram HV, Expedient preparative isolation, quantification and characterization of limonoids from Neem fruits, Anal. Methods., 5, 5386 - 5391 (2013).
  • Haldar S, Kolet SP And Thulasiram HV, Biocatalysis: Fungi mediated Selective 12 beta- or 17 beta- Hydroxylation on the Basic Limonoid Skeleton, Green Chem., 15, 1311 - 1317 (2013).
  • Daramwar PP, Srivastava PL, Priyadarshini B And Thulasiram HV, Preparative separation of alpha- and beta-santalenes and (Z)-alpha- and (Z)-beta-santalols using silver nitrate-impregnated silica gel medium pressure liquid chromatography and analysis of sandalwood oil, Analyst., 137, 4564 - 70 (2012).
  • Daramwar PP, Rincy R, Niloferjahan S, Krithika R, Gulati A, Yadav A, Sharma R And Thulasiram HV, Transformation of (±)-lavandulol and (±)-tetrahydrolavandulol by a fungal strain Rhizopus oryzae, Bioresource Technol., 115, 70 - 74 (2012).
  • Thulasiram HV, Erickson HK And Poulter CD, A Common Mechanism for Branching, Cyclopropanation, and Cyclobutanation Reactions in the Isoprenoid Biosynthetic Pathway, J. Am. Chem. Soc., 130, 1966 - 1971 (2008).
  • Thulasiram HV, Erickson HK And Poulter CD, Chimeras of Two Isoprenoid Synthases Catalyze All Four Coupling Reactions in Isoprenoid Biosynthesis, Science., 316, 73 - 76 (2007).

Research Interest

  • Chemical Biology
  • NATURAL Products
  • Enzymes as Reagents in Organic Synthesis
  • Organic Chemistry

Contact Details

Office: 112, Main Building
Division of Organic Chemistry
CSIR-National Chemical Laboratory
Dr. Homi Bhabha Road
Pune 411008, India
Phone  +91 20 2590 2478
Fax +91 20 2590 2629
E-mail hv.thulasiram@ncl.res.in