Mahesh D. Patil

Mahesh D. Patil

Chemical Engineering & Process Development

About Me

Enzyme-mediated processes are well-recognized for asymmetric transformations, because they can be conducted under mild conditions, and as part of short synthetic routes. The generation of fewer byproducts while avoiding the use of toxic reagents, and the excellent chemo-, region-, and stereo-selectivity exhibited by enzymes can give biocatalytic routes a considerable advantage over traditional abiotic catalysis.  The ever-increasing demands of enzyme-mediated synthesis of industrially important compounds are being elegantly met by engineered biocatalysts. In relation to the downstream processing of biological products, even the modest improvements in the process can mean the difference between commercial success and failure. Despite the availability of various approaches, an effective method for the purification of a biomolecule of interest from numerous intracellular products is a major challenge at all levels from laboratory to large scale. Proteins exhibit the diverse range of characteristics with respect to size, charge, hydrophobicity, etc and therefore purification of a homogeneous protein represents about 60-70% of the total operating cost of the bioprocesses. No single method provides a universal solution for the process of purification of a molecule of interest from milliard of intracellular products.

The research efforts of our lab are dedicated towards upstream and downstream processing and/or analytical characterization of recombinant proteins like antibodies, and industrially important enzymes/ proteins and engineering of various biocatalysts such as transaminases, imine reductases, amine dehydrogenases, monoamine oxidases, oxidoreductases, etc and their application for the synthesis of industrially important chemicals, and pharmaceuticals.

Professional Experience

Research Areas:

-        Biocatalysis and Biotransformation

-        Bioprocess Engineering

-        Downstream Processing

-        Protein Engineering

Professional Experience and Education:

Sept. 2023 - : Scientist- CSIR-National Chemical Laboratory (CSIR-NCL), Pune

Oct. 2021- Sept 2023: M. K. Bhan Young Researcher, DBT- Center of Innovative and Applied Bioprocessing (CIAB), Mohali

Sept 2017- Feb. 2020: Postdoctoral Fellow, Protein Engineering Laboratory, Konkuk University, Seoul, South Korea (with Prof. Hyungdon Yun)

Aug 2012- July 2027: Ph.D, Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research (NIPER), Mohali (with Prof. U. C. Banerjee)

Aug 2010- July 2012: M. Tech (Pharm.), Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research (NIPER), Mohali


Selected Publications

  • Hee-Wang Yoo, Hyunsang Jung, Sharad Sarak, Ye Chan Kim, Beom Gi Park, Byung-Gee Kim, Mahesh D. Patil And Hyungdon Yun, Multi-Enzymatic Cascade Reactions With Escherichia Coli-Based Modules For Synthesizing Various Bioplastic Monomers From Fatty Acid Methyl Esters, Green Chemistry., 24, 2222 - 2231 (2022), DOI:10.1039/D1GC04532F (IF- 11.034).
  • Mahesh D. Patil, # Amol D. Pagar, # Dillon T. Flood, Tae Hyeon Yoo, Philip E. Dawson And Hyungdon Yun, Recent Advances in Biocatalysis with Chemical Modification and Expanded Amino Acids Alphabet, Chemical Reviews., 121, 6173 - 6245 (2021), DOI:10.1021/acs.chemrev.0c01201 (IF-72.087) # Equal contribution.
  • Sharad Sarak, Sihyong Sung, Hyunwoo Jeon, Mahesh D Patil, Taresh P Khobragade, Amol D Pagar, Philip E Dawson And Hyungdon Yun, An Integrated Cofactor/Co?Product Recycling Cascade for the Biosynthesis of Nylon Monomers from Cycloalkylamines, Angewandte Chemie International Edition., 60, 3481 - 3486 (2020), DOI:10.1002/anie.202012658 (IF-16.823).
  • Mahesh D Patil, # Sanghan Yoon, # Sharad Sarak, Hyunwoo Jeon, Geon-Hee Kim, Taresh P Khobragade, Sihyong Sung And Hyungdon Yun, Deracemization of Racemic Amines to Enantiopure (R)- and (S)-amines by Biocatalytic Cascade Employing w-Transaminase and Amine Dehydrogenase, ChemCatChem., 11, 1898 - 1902 (2019), DOI:10.1002/cctc.201900080 (IF-5.501); #Equal authorship.
  • Geon-Hee Kim, Hyunwoo Jeon, Taresh P. Khobragade, Mahesh D Patil, Sihyong Sung, Sanghan Yoon, Yumi Won, In Suk Choi And Hyungdon Yun, Enzymatic synthesis of sitagliptin intermediate using a novel w-transaminase, Enzyme and Microbial Technology., 120, 52 - 60 (2019), DOI:10.1016/j.enzmictec.2018.10.003 (IF-3.705).
  • Mahesh D. Patil, Gideon Grogan, Andreas S. Bommarius And Hyungdon Yun, Oxidoreductase-Catalyzed Synthesis of Chiral Amines, ACS Catalysis., 8, 10985 - 11015 (2018), DOI:10.1021/acscatal.8b02924 (IF-13.700).
  • Sihyong Sung, Hyunwoo Jeon, Sharad Sarak, Md Murshidul Ahsan, Mahesh D. Patil, Wolfgang Kroutil, Byung-Gee Kim And Hyungdon Yun, Parallel anti-sense two-step cascade for alcohol amination leading to w-amino fatty acids and a,w-diamines, Green Chemistry., 20, 4591 - 4595 (2018), DOI:10.1039/C8GC02122H (IF-11.034).
  • Mahesh D. Patil, Manoj J. Dev, Sujit Tangadpalliwar, Gopal Patel, Prabha Garg, Yusuf Chisti And Uttam Chand Banerjee, Ultrasonic disruption of Pseudomonas putida for the release of arginine deiminase: Kinetics and predictive models, Bioresource Technology., 233, 74 - 83 (2017), DOI:10.1016/j.biortech.2017.02.074 (IF-11.4).

Research Interest

  • Bio-chemical AND Biological Engineering

Contact Details

Dr. Mahesh D. Patil


Biochemical & Biological Engineering Building,

Chemical Engineering and Process Development Division

National Chemical Laboratory

Dr. Homi Bhabha Road

Pune 411008, India

Phone:  +91 20 2590 3060

Mobile: +91 9766012203