About Me
Enzyme-mediated
processes are well-recognized for asymmetric transformations, because they can
be conducted under mild conditions, and as part of short synthetic routes. The
generation of fewer byproducts while avoiding the use of toxic reagents, and
the excellent chemo-, region-, and stereo-selectivity exhibited by enzymes can
give biocatalytic routes a considerable advantage over traditional abiotic catalysis.
The ever-increasing demands of
enzyme-mediated synthesis of industrially important compounds are being
elegantly met by engineered biocatalysts. In relation to the downstream
processing of biological products, even the modest improvements in the process
can mean the difference between commercial success and failure. Despite the
availability of various approaches, an effective method for the purification of
a biomolecule of interest from numerous intracellular products is a major
challenge at all levels from laboratory to large scale. Proteins exhibit the diverse
range of characteristics with respect to size, charge, hydrophobicity, etc and
therefore purification of a homogeneous protein represents about 60-70% of the
total operating cost of the bioprocesses. No single method provides a universal
solution for the process of purification of a molecule of interest from
milliard of intracellular products.
The
research efforts of our lab are dedicated towards upstream and downstream
processing and/or analytical characterization of recombinant proteins like
antibodies, and industrially important enzymes/ proteins and engineering of
various biocatalysts such as transaminases, imine reductases, amine dehydrogenases,
monoamine oxidases, oxidoreductases, etc and their application for the
synthesis of industrially important chemicals, and pharmaceuticals.