Mahesh J Kulkarni

Mahesh J Kulkarni

Biochemical Science Division

About Me

Diabetes and its associated complications are becoming global public health problems and posing a serious challenge in disease management. Advanced Glycation End products (AGEs) are implicated in development of insulin resistance, as well as in pathogenesis of diabetic complications. In this context, we have developed methods to quantify plasma AGEs, identified risk factors for elevated plasma AGEs, discovered drug molecules that have the potential to be repositioned as glycation inhibitors, and identified the molecular mechanism for glycation induced formation of protease resistant proteins.  The long-term goal is to identify diagnostic markers for various diabetic complications, identify drug targets and develop intervention strategies.

Professional Experience



Education/ Professional Experience

  • PhD (Crop Physiology), University of Agricultural Sciences, Bangalore, 2002.

  • Scientist-consultant, Monsanto Research Center, Bangalore. 2001-2002

  • Research Associate, Center for cellular and Molecular Biology, Hyderabad, 2003-2004

  • Post doctoral Fellow, Genome Institute  of Singapore, 2004           

  • Scientist, Proteomics, CSIR-National Chemical Laboratory, Pune, 2004 to date

  • Raman Research Fellow,  University of Turku, Finland, 2016




Awards/ Recognition/Membership

•       Elected as Fellow of Maharashtra Academy of Sciences, 2016

•       Elected as Secretary, Proteomics Society, India from 2016.

•       Raman Research Fellow, 2015

•       Editorial Member, Journal of Proteins and Proteomics, (Proteomics Society, India, Journal)

•       NCL Research Foundation - Scientist of the year 2010

•       Life member of Indian Society for Mass spectrometry.

 




Publications (60):


 

1.      Mary S, Kulkarni MJ*, Joshi S, Mehendale S, Giri AP (2017). Tubulointerstitial nephritis antigen-like 1 protein is downregulated in the placenta of pre-eclamptic women. Clinical Proteomics (in press)


2.      Kazi RS,  Banarjee RM,  Deshmukh AB,   Patil GV,  Jagadeeshaprasad MG, Kulkarni MJ*. Glycation inhibitors extend yeast chronological lifespan by reducing advanced glycation end products and by back regulation of proteins involved in mitochondrial respiration. Journal of Proteomics. http://dx.doi.org/10.1016/j.jprot.2017.01.015

 

3.      Mary S, Kulkarni MJ*, Malakar D, Joshi S, Mehendale S, Giri AP (2017). Placental proteomics provides insights into pathophysiology of pre-eclampsia and predicts possible markers inplasma. Journal of Proteome Research. (in press)

 

4.      Batkulwar KB, Jana A, Godbole RK, Sengupta N And Kulkarni MJ*.  Hydralazine inhibits Amyloid beta (Aß) aggregation, glycation and ameliorates Aß1-42 induced neurotoxicity., RSC Advances., , (2016), DOI:10.1039/C6RA20225J.


5.      Jana A, Batkulwar KB, Kulkarni MJ*,  Sengupta N*. Glycation induces conformational changes in amyloid-ß peptide and enhances its aggregation propensity: Molecular Insights.  Physical Chemistry Chemical Physics DOI: 10.1039/c6cp05041g

 

6.      Vannuruswamy G, Jagadeeshaprasad MG, Kashinath K, Kesavan SK, Bhat S, Korwar AM, Chougale AD, Boppana R, Reddy DS*, Kulkarni MJ*. Molecules with O-acetyl group protect protein glycation by acetylating lysine residues. RSC Adv., 2016, 6, 65572

 

7.      Bhat S, Jagadeeshaprasad MG, Patil YR, Shaikh ML, Regin BS, Mohan V, Giri AP, Balasubramanyam M, Boppana R, Kulkarni MJ*. Proteomic insight reveals elevated levels of albumin in circulating immune complexes in diabetic plasma. Mol Cell Proteomics. 2016 Jun;15(6):2011-20

 

8.      Jagadeeshaprasad MG, Batkulwar KB, Meshram NN, Tiwari S, Korwar AM,  Unnikrishnan AG,  Kulkarni MJ*. Targeted quantification of N-1-(carboxymethyl) valine and N-1-(carboxyethyl) valine peptides of β-hemoglobin for better diagnostics in diabetes. Clin Proteom (2016) 13:7.

 

9.      Jangale NM, Devarshi PP, Bansode SB, Kulkarni MJ, Harsulkar AM. Dietary flaxseed oil and fish oil ameliorates renal oxidative stress, protein glycation, and inflammation in streptozotocin-nicotinamide-induced diabetic rats. J Physiol Biochem. 2016 Jun;72(2):327-36.


 10.   Priyanka Singh, Ramesha Jayaramaiah, Sachin Agawane, Vannuruswamy Garikapati, Arvind Korwar, Atul Anand, Vitthal Dhayude, Mahemud Shaikh, Rakesh Joshi, Ramanamurthy Boppana, Mahesh Kulkarni, Hirekodathakallu Thulasiram, and Ashok Giri.  Potential Dual Role of Eugenol in Inhibiting Advanced Glycation End Products in Diabetes: Proteomic and Mechanistic Insights Scientific Reports. 2016 Jan 7;6:18798. doi: 10.1038/srep18798.


11.   Chilukuri H,  Kolekar YM, Bhosle GS, Godbole RK, Kazi RS, Kulkarni MJ*,  and Moneesha Fernandes,* N-(3-aminoalkyl)proline derivatives with potent antiglycation activity.  RSC Adv., 2015, 5, 77332.

 

12.   Rapole S, Mahesh J. Kulkarni, Thakur SS & Sengupta S. Next Generation Proteomics Tools. Nature India Special Issue.2015 18-20,doi: 10.1038/nindia.2015.116

 

13.   Korwar AM, Vannuruswamy G, Jagadeeshaprasad MG, Ramesha JH. Bhat S, Regin BS, Ramaswamy S, Giri AP, Mohan V, Balasumbramanyam M and Kulkarni MJ*. Development of diagnostic fragment ion library for glycated peptides of Human serum albumin: Targeted quantification in prediabetic, diabetic and microalbuminuria plasma by PRM, SWATH and MSE. Molecular and Cellular Proteomics. 2015 Aug;14(8):2150-9

 

14.   Bansode SB, Batkulwar KB, Warkad S,  Jana A, , Sengupta N, Kulkarni MJ*. Tolbutamide induces conformational change and promotes albumin glycation. RSC Advances, 2015, 40070-40075 


15.   A, Korwar AM, Kulkarni MJ Joshi SP* Recent development of plant products with antiglycation activity: a review. Chinchansure. RSC Advances., 2015, 5, 31113-31138.


16.   Kolekar YM, Vannuruswamy G, Bansode SB, Santhakumari B, Thulasiram HV, Kulkarni MJ*. Investigation of Antiglycation Activity of Isoprenaline. RSC Advances., 2015,5, 25051-25058.


17.   Golegaonkar SB,  Tabreza SS,  Pandita A, Shalini SA, Jagadeesha Prasad MG, Bansode SB,  Gopalan SC, Kulkarni MJ*, and Mukhopadhyay A*. Rifampicin reduces Advanced Glycation End products and activates DAF-16 to increase life span in Caenorhabditis elegans. (2015). Aging Cell. 2015 Jun;14(3):463-73.


18.   Batkulwar KB, Bansode SB, Patil GV, Godbole RK, Kazi RS, Chinnatambi S, Shanmugam D, Kulkarni MJ*. "Investigation of Phosphoproteome in RAGE signaling. Proteomics. 2015 Jan;15(2-3):245-59


19.   Tiwari S, Bothale M, Kulkarni MJ, Basu R, Basu A, Hasan I, Unnikrishnan AG. Association between Serum Albumin and HbA1c in Asian Indian Subjects. Ind J Endocrinol Metabol, 2014, 2015 Jan-Feb;19(1):52-5.


 20.   Bansode SB, Jana A, Batkulwar KB, Warkad S, Joshi RS, Sengupta N*, Kulkarni MJ*. Molecular Investigations of Protriptyline as a Multi-Target Directed Ligand in Alzheimer's Disease. PLoS One. 2014 Aug 20;9(8):e105196. doi: 10.1371/journal.pone.0105196.



21.   Mukherjee S, Jagadeeshaprasad M , Banerjee T, Ghosh S,  Biswas M, Dutta S Kulkarni MJ, Patari S, Bandyopadhyay A. Proteomic analysis of human plasma in Chronic Rheumatic Mitral Stenosis reveals proteins involved in the Complement and Coagulation cascade.  Clinical Proteomics 2014, 11:35  doi:10.1186/1559-0275-11-35


 22.   Mohammad N, Malvi P, Meena AS, Singh SV, Chaube B, Vannuruswamy G, Kulkarni MJ, Bhat MK. Cholesterol depletion by methyl-beta-cyclodextrin augments tamoxifen induced cell death by enhancing its uptake in melanoma. Mol Cancer. 2014 Sep 1;13(1):204.


 23.   Singh P, Ramesha HJ, Sarate P, Thulasiram HV, Kulkarni MJ,  Giri AP.  Insecticidal potential of defense metabolites from Ocimum kilimandscharicum against Helicoverpa armigera.. PLoS One. 2014 Aug 6;9(8):e104377. doi: 10.1371/journal.pone.0104377.


 24.   Sacheti P, Patil R, Dube A, Bhonsle H, Thombre D, Marathe S, Vidhate R, Wagh P, Kulkarni MJ.  Rapole S, Gade W. Proteomics of arsenic stress in the gram-positive organism Exiguobacterium sp. PS NCIM 5463. Appl Microbiol Biotechnol. 2014 Aug;98(15):6761-73.


 25.   Bhat S, Mary S, Banarjee R, Giri AP, Kulkarni MJ*. Immune response to chemically modified proteome. Proteomics Clin Appl. 2014 Feb;8(1-2):19-34.


 26.   Kesavan SK, Bhat S, Golegaonkar SB, Jagadeeshaprasad MG, Deshmukh AB, Patil HS, Bhosale SD, Shaikh ML, Thulasiram HV, Boppana R, Kulkarni MJ*.  Proteome wide reduction in AGE modification in streptozotocin induced diabetic mice by hydralazine mediated transglycation. Scientific Reports. 2013 Oct 15;3:2941. doi: 10.1038/srep02941.


 27.   Joglekar M, Panaskar S, Chougale A, Kulkarni M,  Arvindekar A. A novel mechanism for antiglycative action of limonene through stabilization of protein conformation Mol Biosyst. 2013 Oct;9(10):2463-72


 28.   Bhonsle HS,  Korwar AM, Suresh KK, Bhosale SD Bansode SB, Kulkarni MJ*. 'Zoom in' - A targeted database search for identification of glycation modifications analyzed by untargeted tandem mass spectrometry" Eur J Mass Spectrom. Volume 18 Issue 6, Pages 475–481 (2012)


 29.   Kulkarni MJ*, Korwar AM, Mary S, Bhonsle HS, Giri AP. Glycated proteome: from reaction to intervention. Proteomics Clin Appl. 2013 Jan;7(1-2):155-70.


30.   Bansode SB, Chougale AD, Joshi RS, Giri AP, Bodhankar SL, Harsulkar AM, Kulkarni MJ*. Proteomic analysis of protease resistant proteins in the diabetic rat kidney. Mol Cell Proteomics. 2013 Jan;12(1):228-36


 31.   Korwar AM, Bhonsle HS, Ghole VS, Gawai KR, Koppikar CB and Kulkarni MJ*. Proteomic profiling and interactome analysis of ER positive/ HER2/neu negative invasive ductal carcinoma of the breast: Towards proteomics biomarkers.  OMICS. 2013 Jan;17(1):27-40


 32.   Bhonsle HS,  Korwar AM, Chougale AD  Kote SS, Dhande NL,  Shelgikar KM,  Kulkarni MJ*. Proteomic study reveals downregulation of apolipoprotein A1 in plasma of poorly controlled diabetes: A pilot study. Mol Med Report. 2013 Feb;7(2):495-8.



33.   Nivedita M. Jangale, Prasad P. Devarshi, Aarti A. Dubal, Arvindkumar E. Ghule, Soumya J. Koppikar, Subhash L. Bodhankar, Ashok D. Chougale, Mahesh J. Kulkarni, Abhay M. Harsulkar. Dietary flaxseed oil and fish oil modulates expression of antioxidant and inflammatory genes with alleviation of protein glycation status and inflammation in liver of streptozotocin–nicotinamide induced diabetic rats.  Food Chemistry

Selected Publications

  • Jagadeeshaprasad MG, Subramani V, Unnikrishnan AG And Kulkarni MJ*. , Albumin abundance and its glycation Status Determine Hemoglobin Glycation, ACS Omega., , (2018), DOI:dx.doi.org/10.1021/acsomega.8b01702.
  • Bhat S, Jagadeeshaprasad MG, Patil YR, Shaikh ML, Regin BS, Mohan V, Giri AP, Balasubramanyam M, Boppana R And Kulkarni MJ, Proteomic insight reveals elevated levels of albumin in circulating immune complexes in diabetic plasma, Molecular Cellular Proteomics., 6, (2016), DOI:mcp.M116.058008.
  • Golegaonkar SB, Tabreza SS, Pandita A, Shalini SA, Jagadeesha Prasad MG, Bansode SB, Gopalan SC, Kulkarni MJ* And and Mukhopadhyay A*. , Rifampicin reduces Advanced Glycation End products and activates DAF-16 to increase life span in Caenorhabditis elegans, Aging Cell., 14(3), 463 - 73 (2015).
  • Batkulwar KB, Bansode SB, Patil GV, Godbole RK, Kazi RS, Chinnatambi S, Shanmugam D And Kulkarni MJ*. , Investigation of Phosphoproteome in RAGE signaling. Jan;15(2-3):245-59, Proteomics., 15, 245 - 59 (2015).
  • Rapole S, Mahesh J. Kulkarni* And Thakur SS & Sengupta S, Next Generation Proteomics Tools, Nature India., , 18 - 20 (2015), DOI:10.1038/nindia.2015.116.
  • Bansode SB, Jana A, Batkulwar KB, Warkad S, Joshi RS, Sengupta N* And Kulkarni MJ*. , Molecular Investigations of Protriptyline as a Multi-Target Directed Ligand in Alzheimer's Disease. , Plos One., Aug 20;9(8), e105196 (2014), DOI:doi: 10.1371/journal.pone.0105196.
  • Kesavan SK, Bhat S, Golegaonkar SB, Jagadeeshaprasad MG, Deshmukh AB, Patil HS, Bhosale SD, Shaikh ML, Thulasiram HV, Boppana R And Kulkarni MJ*. , Proteome wide reduction in AGE modification in streptozotocin induced diabetic mice by hydralazine mediated transglycation. , Scientific Reports., 15;3, 2941 (2013), DOI:doi: 10.1038/srep02941.
  • Bansode SB, Chougale AD, Joshi RS, Giri AP, Bodhankar SL, Harsulkar AM And Kulkarni MJ*. , Proteomic analysis of protease resistant proteins in the diabetic rat kidney, Mol Cell Proteom., 12 (1), 228 - 36 (2013).
  • Bhonsle HS, Korwar AM, Kote SS, Golegaonkar SB, Chougale AD, Shaik ML, Dh, e NL, Giri AP, Shelgikar KM, Boppana R And Kulkarni MJ, Low plasma albumin levels are associated with increased plasma protein glycation and HbA1c in diabetes, J Proteome Res., 11, 1391 - 1396 (2012).

Research Interest

  • Chemical Biology
  • Mass Spectrometry
  • Metabolomics
  • Proteomics and Metabolomicsy
  • Biological Mass Spectrometry
  • Drug Discovery for AGE reduction

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