Welcome to the Jha Lab
Proteins are functional entities in all living systems. They perform numerous functions including catalysis of chemical reactions, transport of ions and molecules, coordination of motion, provision of mechanical support, generation and transmission of nerve impulses, and control of growth and differentiation. Inside living cells,a protein molecule is synthesized as a linear unfolded polypeptide chain which must fold to a unique three dimensional structure in order to be functional. An error in this process leads to fatal diseases. Our research is focused on understanding the rules which govern the folding, unfolding and aggregation of proteins. We employ a highly interdisciplinary approach to address these problems by combining the tools of biochemistry, molecular biology, theory, and advance spectroscopy, including time-resolved fluorescence resonance energy transfer (TR-FRET) technique, time-resolved infrared spectroscopy and thiol-disulfide exchange methodology coupled to mass spectrometry. The long term goal of our research is to understand how the structure and dynamics of biological macromolecules contribute to biological function, how they are altered in disease and how they can be exploited for therapeutic benefit.