Research Topic:

Our interest is chemistry and biology of natural products. The main focus of our research is to explore the natural products with special emphasis on isolation structural characterization, bioactivity, biosynthesis, modification and mechanistic insights for their biosynthesis. A multidisciplinary approach involving concepts of organic chemistry, analytical chemistry, biochemistry, molecular biology, and microbiology are employed to address fundamental problems at the interface of chemistry and biology

Our lab is well equipped for conducting the research in multidisciplinary area of Chemical Biology.

1. Biosynthesis of isoprenoids

       Genome / transcriptome mining for the genes which encodes enzymes involved in the isoprenoids biosynthesis

       Characterization of enzymes in the isoprenoid biosynthetic pathway and establish the mechanisms they utilize for catalysis

       Synthesize and evaluate alternate substrates and inhibitors for enzymes.

       Evaluate the role of catalytic site residues by site-directed mutagenesis.

       Use of recombinant DNA technology to synthesize enzymes for core skeletons of biologically and commercially important secondary metabolites

       Study X-ray structures of enzyme-inhibitor complexes

2.  Biocatalysis/Biotransformation

Utilization of the nature’s catalysts for the synthesis/modification of biologically and commercially important organic molecules

       Screening of microorganisms (isolated/culture collection)/isolated enzymes for novel and useful transformations

       Enzyme expression, purification, characterization, stability studies and immobilization

       Use of recombinant DNA technology for structure-based engineering or to synthesize the chimeric enzymes with new activity

       Utilizing the microbes as “model for mammalian metabolism”

3. The search for bioactive substances (isolation, structure elucidation and modifications)

  • Isolation, structural characterization and bioactivity of natural product small molecules from plant and microorganisms
  • Developing effective strategies to discover and exploit new small molecules is integral to the success of future drug discovery efforts



Dr. H. V. Thulasiram
Division of Organic Chemistry
National Chemical Laboratory
Dr. Homi Bhabha Road
Pune 411008, INDIA
Ph. No. 91-20-2590 2478
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