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Dr. Mahesh J Kulkarni Senior Principal Scientist and Chair Biochemical Sciences Division CSIR-National Chemical Laboratory Pune-411008, India E-mail: mj.kulkarni@ncl.res.in Ph. No. +91-20-25902541, Mobile. +91-9890454550 http://academic.ncl.res.in/mj.kulkarni ORCID: https://orcid.org/0000-0003-3932-9092
About me Dr. Mahesh J Kulkarni is a Senior Principal Scientist and Chair, Biochemical Sciences Division at CSIR National Chemical Laboratory Pune. He is instrumental in setting up a mass spectrometry and proteomics facility at NCL. He has guided 15 Ph.D. students hitherto and has published 111 articles in reputed journals like Molecular Cellular Proteomics, Journal of Proteome Research, Proteomics, RSC Advances, Molecular Biosystems, Journal of Proteomics, Scientific Reports, Aging Cell, Elife, BBA Molecular Basis of diseases etc. He has led several important projects as a Principal investigator. Dr. Kulkarni has contributed significantly to understanding protein glycation in diabetes by using mass spectrometric and proteomics approaches. He is also working on developing value-added biosimilars and characterization of biotherapeutics and therapeutic mAbs. He is an editorial member of reputed journals such as Clinical Proteomics Journal, and Journal of Proteins and Proteomics. He has been elected Fellow of Maharashtra Academy of Sciences in 2016. He was Raman Research Fellowship to visit University of Turku, Finland, 2016.
Education Qualification
1. Sheikh AB , Jathar SM, Kulkarni MJ*. Characterization of S-homocysteinylation of human Insulin and its implications in diabetes. Protein J. (2025, in press)
2. Patil YR, Tiwari S, · Momin AA, Unnikrishnan AG, Kulkarni MJ*. The Proteome Profile of Stress Test Assessed Cardiovascular Disease Risk-Prone Diabetic Subjects. Journal of Cardiovascular Translational Research (2025, in press)
3. Patil YR, Tiwari S, · Momin AA, Unnikrishnan AG, Kulkarni MJ*. Autoantibodies against Nε-carboxymethyl lysine and methylglyoxal modified albumin are associated with cardiovascular risk in type 2 diabetes. International Journal of Diabetes in Developing Countries.2025 . doi.org/10.1007/s13410-024-01435-0
4. Ambekar AS, Naredi N, Malakar D, Vashum Y, Misra P, Kulkarni M*. Early and advanced glycation end product analysis from women with PCOS on metformin. Reprod Biol. 2025 Jan 14;25(1):100993. doi: 10.1016/j.repbio.2024.100993.
5. Rajesh S, Jathar S, Banarjee R, Sharma M, Palkar S, Shankar SS, Kulkarni MJ*. A simple freeze-thaw based method for efficient purification of recombinant human proinsulin from inclusion bodies. Protein Expr Purif. 2024 Dec 14:106645. doi: 10.1016/j.pep.2024.106645. Epub ahead of print. PMID: 39681151.
6. Sonwane BP, Raut P, Chitalkar J, Godbole S, Sabnis S, Gupta J, Santhakumari B, Deshpande MV, Kulkarni MJ*, Yoga therapy Attenuates the Progression of Diabetes – Insights from Proteomics and Metabolomics Analysis. International Journal of Yoga 17 (3), 163-174.
7. Todkari IA, Chaudhary P, Kulkarni MJ, Ganesh KN. Supramolecular polyplexes from Janus peptide nucleic acids (bm-PNA-G5): self-assembled bm-PNA G-quadruplex and its tetraduplex with DNA. Org Biomol Chem. 2024 Aug 22;22(33):6810-6821. doi: 10.1039/d4ob00968a. PMID: 39113548.
8. Ghosh A, Jani V, Sonavane U, Naphade AN, Joshi R, Kulkarni MJ, Giri AP*. The multi-dimensional impact of captopril modification on human serum albumin. Int J Biol Macromol. 2024 Jun 20;274(Pt 2):133289.
9. Kulsange S, Sharma M, Sonawane B, Jaiswal MR, Kulkarni M, Santhakumari B. SWATH-MS reveals that Bisphenol A and its analogs regulate pathways leading to disruption in Insulin signaling and fatty acid metabolism. Food Chem Toxicol. 2024 Apr 21:114667. doi: 10.1016/j.fct.2024.114667.
10. Shaikh RQ, Das S, Chaurasiya AH, Ashtamy MG, Sheikh AB, Fernandes M, Tiwari S, Unnikrishnan AG, Kulkarni MJ*. Discovery of free glycated amines and glycated urea in diabetic plasma: Potential implication in diabetes. ACS Omega, 2024 https://doi.org/10.1021/acsomega.4c01772.
11. Chinnathambi S, Sonawane SK, Hariharakrishnan Chidambaram MC, T Shrivatsa, Kulkarni MJ. Dual Modification of Tau by Pseudophosphorylation and Glycation Does Not Enhance Amorphous Aggregation. Cellular Physiology and Biochemisty. 2024 DOI:10.33594/000000693
12. Shankar SS, Banarjee R, Jathar SM, Rajesh S, Ramasamy S, Kulkarni MJ*. De novo structure prediction of meteorin and meteorin-like protein for identification of domains, functional receptor binding regions, and their high-risk missense variants. J Biomol Struct Dyn. 2023 Jun 8:1-15. doi: 10.1080/07391102.2023.2220804.
13. Chaurasiya AH, Khilari AA, Kazi A, Jaiswal MR, Gouri Bhoite GM, Padwal MK, Momin AA*, Shanmugam D*, and Kulkarni MJ* Nanopore Sequencing of RAGE Gene Polymorphisms and Their 2 Association with Type 2 Diabetes. ACS Omega. 2023 Jul 10;8(29):25727-25738.
14. Chaurasiya AH, Jaiswal M; Bayatigeri S; Kahar S; Tiwari S, Unnikrishnan AG, Kulkarni MJ*. Elevated level of glycated KQTALVELVK peptide of albumin is associated with the risk of diabetic nephropathy. ACS Omega (2023) ACS Omega. 2023 Jun 1;8(23):20654-20660.
15. Bansode S, Singh PK, Tellis M, Chugh A, Deshmukh N, Gupta M, Verma S, Giri A, Kulkarni M, Joshi R, Chaudhry D*. A comprehensive molecular and clinical investigation of approved anti-HCV drugs repurposing against SARS-CoV-2 infection: A Glaring Gap between Benchside and Bedside Medicine. Vaccines 2023, 11(3), 515.
16. Garg A, Alam M, Bai S, Dandawate M, Kumari N, Gupta S, Agrawal U, Nagarajan P, Reddy DS, Kulkarni MJ, Mukhopadhyay A*. Protective effects of rifampicin and its analog rifampicin quinone in a mouse model of obesity-induced type 2 diabetes. ACS Pharmacology and Translational Science 2023 Jan 12;6(2):253-269.
17. Pandya VK, Shankar S, Sonawane B, Rajesh S, Rathore R, Kumaran S, Kulkarni MJ*. Mechanistic insights on anserine hydrolyzing activities of human carnosinases. Biochim Biophys Acta Gen Subj 2023 Mar;1867(3):130290)
18. Patil G, Kulsange S, Kazi R, Chirmade T, Kale V, Mote C, Aswar M, Koratkar S, Agawane S, Kulkarni M*. Behavioral and proteomic studies reveal methylglyoxal activate pathways associated with Alzheimer’s disease. ACS Pharmacology and Translational Science 2022 Dec 27;6(1):65-75.
19. Vennapusa AR, Agarwal S, Hm HR, Aarthy T, Babitha KC, Thulasiram HV, Kulkarni MJ, Melmaiee K, Sudhakar C, Udayakumar M. Vemanna RS, Stacking herbicide detoxification and resistant genes improves glyphosate tolerance and reduces phytotoxicity in tobacco (Nicotiana tabacum L.) and rice (Oryza sativa L.) Plant Physiology and Biochemistry (2022) 189, 126-138
20. Ghosh A, Patil AB, Chirmade TP, Jathar SM, Bhambure RS, Sengupta D, Giri AP, Kulkarni MJ* Investigation of captopril-insulin interaction by mass spectrometry and computational approaches reveal captopril induces structural changes in insulin (2022) ACS Omega 2022 Jun 30;7(27):23115-23126.
21. Singh P, et al. Kulkarni M, et al., Sengupta S*, Dash D*. A machine learning-based approach to determine infection status in recipients of BBV152 (Covaxin) whole-virion inactivated SARS-CoV-2 vaccine for serological surveys. Comput Biol Med. 2022 Apr 25;146:105419. doi: 10.1016/j.compbiomed.2022.105419
22. Rana R*, Chauhan K, Gautam P, Kulkarni M, Banarjee R, Chugh P,. Chhabra SS, Acharya R, Kalra SK, Gupta A, Jain S, Ganguly NK. Plasma-Derived Extracellular vesicles reveal Galectin-3 binding protein as Potential Biomarker for Early Detection of Glioma. Front Oncol. 2021 Nov 26;11:778754. doi: 10.3389/fonc.2021.778754.
23. Routaraya CB, Choudharia V, Prakasha D, Patil R, Jagtap S, Baid S, Kulkarni MJ, Kuchipudi SV, Kalpana Pai* Quantitative proteomic analysis reveals differential modulation of crucial stage specific proteins during promastigote to amastigote differentiation in Leishmania donovani", J Proteins Proteom (2021). https://doi.org/10.1007/s42485-021-00080-z
24. Kaur T, Shukla BN, Yadav V, Kulkarni MJ, and Alka Rao*. Comparison of glycoprofiles of Rituximab versions licensed for sale in India and an analytical approach for quality assessment. J Proteomics. 2021 May 17:104267. doi: 10.1016/j.jprot.2021.104267)
25. Naushin S, et al., Kulkarni M, et al. Sengupta S. Insights from a Pan India Sero-Epidemiological survey (Phenome-India Cohort) for SARS-CoV2. Elife. 2021 Apr 20;10:e66537. doi: 10.7554/eLife.66537.
26. Joshi RS*, Giri AP, Kulkarni MJ, Gupta M, Verma S, Chaudhry D, Deshmukh N and Chugh A. Antiviral drug prioritization for COVID-19 management based on rational selection. Current Science, 2021, VOL. 120, NO. 9, 1464.
27. Tiwari V, Mishra A, Singh S, Kumar Mishra S, Kumari Sahu K, Parul, Kulkarni MJ, Shukla R, Shukla S*. Protriptyline improves spatial memory and reduces oxidative damage by regulating NFκB-BDNF/CREB signaling axis in streptozotocin-induced rat model of Alzheimer's disease. Brain Res. 2021 Jan 7:147261. doi: 10.1016/j.brainres.2020.147261
28. Walke PB, Bansode SB, More NP, Chaurasiya AH, Joshi RS, Kulkarni MJ*.Molecular investigation of glycated insulin-induced insulin resistance via insulin signaling and AGE-RAGE axis. Biochim Biophys Acta Mol Basis Dis. 2021;1867(2):166029. doi: 10.1016/j.bbadis.2020.166029.
29. Pradhan G, Sneha M, Sonwane BP, Santhakumari B, Rao A*, Kulkarni MJ*. Multiple-parallel-protease digestion coupled with high-resolution mass spectrometry: An approach towards comprehensive peptide mapping of therapeutic mAbs. J Proteomics. 2020 Nov 22:104053. doi: 10.1016/j.jprot.2020.104053
30. Thoduvayil S, Dhandapani G, Brahma R, Rex DA., Mangalaparthi KK, Patel K, Kumar Tennyson MJ, Satheeshkumar PK, Kulkarni MJ, Pinto SM, Keshava Prasad TS, Madanan MG*. Triton X-114 fractionated subcellular proteome of Leptospira interrogans shows selective enrichment of pathogenic and outer membrane proteins in the detergent fraction" Proteomics (doi: 10.1002/pmic.202000170.)
31. Bai S, Chaurasiya AH, Banarjee R, Walke P, Kulkarni MJ*. CD44, a predominant protein in methylglyoxal induced secretome of muscle cells, is elevated in diabetic plasma. ACS Omega (doi.org/10.1021/acsomega.0c01318)
32. Joshi RS*, Jagdale SS, Bansode SB, Shankar SS, Tellis MB, Pandya VK, Chugh A, Giri AP*, Kulkarni MJ*. Discovery of potential multi-target-directed ligands by targeting host-specific SARS-CoV-2 structurally conserved main protease. J Biomol Struct Dyn. 2020 May 5:1-16.
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