CSIR-National Chemical Laboratory Faculty

Mahesh J Kulkarni, PhD Scientist, Proteomics Facility Division of Biochemical Sciences CSIR-National Chemical Laboratory Pune-411008 E-mail: mj.kulkarni@ncl.res.in Ph. No. +91-20-25902541 Mobile. +91-9890454550 Research Interests: Mass spectrometry and Proteomics, Advanced Glycation End Products and Diabetes Our group’s research activity involves developing mass spectrometric application for the proteomic research, as well as to understand the molecular mechanisms of biological process using proteomic approaches. Currently we are working on proteomics of diabetes where in we are trying to elucidate the role of glycated proteins and advanced glycation end products (AGEs) in developing diabetic complication. The long term goal is to identify a diagnostic marker for diabetic complications, identify drug targets and develop intervention strategies. Awards/ Recognition/Membership • NCL Research Foundation - Scientist of the year 2010 • Elected as Young Associate of Maharashtra Academy of Sciences, 2007 • Life member of Indian Society for Mass spectrometry. • Joint Secretary, Proteomics Society, India from 2014 Best 5 papers 1. Rifampicin reduces Advanced Glycation End products and activates DAF-16 to increase life span in Caenorhabditis elegans. Golegaonkar SB, Tabreza SS, Pandita A, Shalini SA, Jagadeesha Prasad MG, Bansode SB, Gopalan SC, Kulkarni MJ*, and Mukhopadhyay A*. (2015). Aging Cell. 2015 Feb 26. doi: 10.1111/acel.12327 (IF 5.9) 2. Molecular Investigations of Protriptyline as a Multi-Target Directed Ligand in Alzheimer's Disease. Bansode SB, Jana A, Batkulwar KB, Warkad S, Joshi RS, Sengupta N*, Kulkarni MJ*. PLoS One. 2014 Aug 20;9(8):e105196. doi: 10.1371/journal.pone.0105196. (IF 3.5) 3. Proteome wide reduction in AGE modification in streptozotocin induced diabetic mice by hydralazine mediated transglycation. Kesavan SK, Bhat S, Golegaonkar SB, Jagadeeshaprasad MG, Deshmukh AB, Patil HS, Bhosale SD, Shaikh ML, Thulasiram HV, Boppana R, Kulkarni MJ*. Sci Rep (Nature Publications). 2013 Oct 15;3:2941. doi: 10.1038/srep02941. (IF 5.0) 4. Proteomic analysis of protease resistant proteins in the diabetic rat kidney. Bansode SB, Chougale AD, Joshi RS, Giri AP, Bodhankar SL, Harsulkar AM, Kulkarni MJ*. Mol Cell Proteomics. 2013 Jan;12(1):228-36 (IF 7.25) 5. Low plasma albumin levels are associated with increased plasma protein glycation and HbA1c in diabetes. Bhonsle HS, Korwar AM, Kote SS, Golegaonkar SB, Chougale AD, Shaik ML, Dhande NL, Giri AP, Shelgikar KM, Boppana R, Kulkarni MJ*. J Proteome Res. 2012 Feb 3;11(2):1391-6. (IF 5.0) Publications (45): 1. Investigation of Antiglycation Activity of Isoprenaline. Kolekar YM, Vannuruswamy G, Bansode SB, Santhakumari B, Thulasiram HV, Kulkarni MJ*. RSC Advances, 2015 (just accepted) 2. Rifampicin reduces Advanced Glycation End products and activates DAF-16 to increase life span in Caenorhabditis elegans. Golegaonkar SB, Tabreza SS, Pandita A, Shalini SA, Jagadeesha Prasad MG, Bansode SB, Gopalan SC, Kulkarni MJ*, and Mukhopadhyay A. (2015). Aging Cell. 2015 Feb 26. doi: 10.1111/acel.12327 3. "Investigation of Phosphoproteome in RAGE signaling" Batkulwar KB, Bansode SB, Patil GV, Godbole RK, Kazi RS, Chinnatambi S, Shanmugam D, Kulkarni MJ*. Proteomics. 2015 Jan;15(2-3):245-59 4. Association between Serum Albumin and HbA1c in Asian Indian Subjects. Tiwari S, Bothale M, Kulkarni MJ, Basu R, Basu A, Hasan I, Unnikrishnan AG. Ind J Endocrinol Metabol, 2014, 2015 Jan-Feb;19(1):52-5. 5. Proteomic analysis of human plasma in Chronic Rheumatic Mitral Stenosis reveals proteins involved in the Complement and Coagulation cascade. Mukherjee S, Jagadeeshaprasad M , Banerjee T, Ghosh S, Biswas M, Dutta S Kulkarni MJ, Patari S, Bandyopadhyay A Clinical Proteomics 2014, 11:35 doi:10.1186/1559-0275-11-35 6. Cholesterol depletion by methyl-beta-cyclodextrin augments tamoxifen induced cell death by enhancing its uptake in melanoma.Mohammad N, Malvi P, Meena AS, Singh SV, Chaube B, Vannuruswamy G, Kulkarni MJ, Bhat MK. Mol Cancer. 2014 Sep 1;13(1):204. 7. Molecular Investigations of Protriptyline as a Multi-Target Directed Ligand in Alzheimer's Disease. Bansode SB, Jana A, Batkulwar KB, Warkad S, Joshi RS, Sengupta N*, Kulkarni MJ*. PLoS One. 2014 Aug 20;9(8):e105196. doi: 10.1371/journal.pone.0105196. 8. Insecticidal potential of defense metabolites from Ocimum kilimandscharicum against Helicoverpa armigera. Singh P, Ramesha HJ, Sarate P, Thulasiram HV, Kulkarni MJ, Giri AP. PLoS One. 2014 Aug 6;9(8):e104377. doi: 10.1371/journal.pone.0104377. 9. Proteomics of arsenic stress in the gram-positive organism Exiguobacterium sp. PS NCIM 5463. Sacheti P, Patil R, Dube A, Bhonsle H, Thombre D, Marathe S, Vidhate R, Wagh P, Kulkarni M, Rapole S, Gade W. Appl Microbiol Biotechnol. 2014 Jun 17. [Epub ahead of print]. 10. Immune response to chemically modified proteome. Bhat S, Mary S, Banarjee R, Giri AP, Kulkarni MJ*. Proteomics Clin Appl. 2014 Feb;8(1-2):19-34. 11. Proteome wide reduction in AGE modification in streptozotocin induced diabetic mice by hydralazine mediated transglycation. Kesavan SK, Bhat S, Golegaonkar SB, Jagadeeshaprasad MG, Deshmukh AB, Patil HS, Bhosale SD, Shaikh ML, Thulasiram HV, Boppana R, Kulkarni MJ*. Sci Rep (Nature Publications). 2013 Oct 15;3:2941. doi: 10.1038/srep02941. 12. A novel mechanism for antiglycative action of limonene through stabilization of protein conformation Joglekar M, Panaskar S, Chougale A, Kulkarni M, Arvindekar A. Mol Biosyst. 2013 Oct;9(10):2463-72 13. 'Zoom in' - A targeted database search for identification of glycation modifications analyzed by untargeted tandem mass spectrometry" Bhonsle HS, Korwar AM, Suresh KK, Bhosale SD Bansode SB, Kulkarni MJ*. Eur J Mass Spectrom. Volume 18 Issue 6, Pages 475–481 (2012) 14. Glycated proteome: from reaction to intervention. Kulkarni MJ*, Korwar AM, Mary S, Bhonsle HS, Giri AP. Proteomics Clin Appl. 2013 Jan;7(1-2):155-70. 15. Proteomic analysis of protease resistant proteins in the diabetic rat kidney. Bansode SB, Chougale AD, Joshi RS, Giri AP, Bodhankar SL, Harsulkar AM, Kulkarni MJ*. Mol Cell Proteomics. 2013 Jan;12(1):228-36 16. Proteomic profiling and interactome analysis of ER positive/ HER2/neu negative invasive ductal carcinoma of the breast: Towards proteomics biomarkers. Korwar AM, Bhonsle HS, Ghole VS, Gawai KR, Koppikar CB and Kulkarni MJ*. OMICS. 2013 Jan;17(1):27-40 17. Proteomic study reveals downregulation of apolipoprotein A1 in plasma of poorly controlled diabetes: A pilot study. Bhonsle HS, Korwar AM, Chougale AD Kote SS, Dhande NL, Shelgikar KM, Kulkarni MJ* . Mol Med Report. 2013 Feb;7(2):495-8. 18. Dietary flaxseed oil and fish oil modulates expression of antioxidant and inflammatory genes with alleviation of protein glycation status and inflammation in liver of streptozotocin–nicotinamide induced diabetic rats. Nivedita M. Jangale, Prasad P. Devarshi, Aarti A. Dubal, Arvindkumar E. Ghule, Soumya J. Koppikar, Subhash L. Bodhankar, Ashok D. Chougale, Mahesh J. Kulkarni, Abhay M. Harsulkar. Food Chemistry, Volume 141, Issue 1, 1 November 2013, Pages 187-195 19. Stress inducible proteinase inhibitor diversity in Capsicum annuum. Mishra M, Mahajan N, Tamhane VA, Kulkarni MJ, Baldwin IT, Gupta VS, Giri AP. BMC Plant Biol. 2012 Nov 16;12(1):217. 20. Arsenomics of Exiguobacterium sp. PS (NCIM 5463) Poonam Sacheti, Hemangi Bhonsle, Rajendra Patil, Mahesh J. Kulkarni, Rapole Srikanth and Wasudev Gade RSC Adv 2013, 3 Issue: 25 Pages: 9705-9713 21. Synergistic action of flavin containing NADH dependant azoreductase and cytochrome P450 monooxygenase in azoaromatics mineralization. Chetan C. Oturkar CC, Othman MA, Kulkarni MJ, Madamwar D, Gawai kR. RSC Adv., 2013,3, 3062-3070. 22. Analysis of AGE modified proteins and RAGE expression in HER2/neu negative invasive ductal carcinoma. Korwar AM, Bhonsle HS, Chougale AD, Kote SS, Gawai KR, Ghole VS, Koppikar CB, Kulkarni MJ*. Biochem Biophys Res Commun. 2012 Mar 16;419(3):490-4. 23. Low plasma albumin levels are associated with increased plasma protein glycation and HbA1c in diabetes. Bhonsle HS, Korwar AM, Kote SS, Golegaonkar SB, Chougale AD, Shaik ML, Dhande NL, Giri AP, Shelgikar KM, Boppana R, Kulkarni MJ*. J Proteome Res. 2012 Feb 3;11(2):1391-6. 24. Dynamic proteome in enigmatic preeclampsia: An account of molecular mechanisms and biomarker discovery. Sheon MS1, Patil GV, Kulkarni VA, Kulkarni MJ*, Joshi SR, Mehendale SS, Giri AP (2012) Proteomics Clin Appl. 2012 Jan;6(1-2):79-90 (Invited review) 25. Effects of arsenite stress on growth and proteome of Klebsiella pneumonia. Daware V, Suresh KK, Kulkarni MJ, Gade WN. Journal of Biotechnology (2012) (in press) 26. Purification and characterization of an antioxidant protein (16 kDa) from Terminalia chebula fruit. Pratibha Srivastava , Hema N. Raut , Renuka S. Wagh , Hemalata M. Puntambekar, Kulkarni MJ. Food Chemistry 131 (2012) 141–148 27. Comparative and chemical proteomic approaches reveal gatifloxacin deregulates enzymes involved in glucose metabolism. Suresh KK, Bhosale SD, Thulasiram HV, Kulkarni MJ*. J Toxicol Sci. 2011;36(6):787-96. 28. Proteomic Analysis of Glycated Proteins from Streptozotocin-Induced Diabetic Rat Kidney. Chougale AD, Bhat SP, Bhujbal SV, Zambare MR, Puntambekar S, Somani RS, Boppana R, Giri AP, Kulkarni MJ*. Mol Biotechnol. 2012 Jan;50(1):28-38. 29. Bioanalytical method development and validation of vildagliptin a novel dipeptidyl peptidase IV inhibitor by RP-HPLC method AB Pharne, B Santhakumari, AS Ghemud, HK Jain, MJ Kulkarni. Int J Pharm Pharm Sci 4 (3), 119-123 30. Interaction of recombinant CanPIs with Helicoverpa armigera gut proteases reveals their processing patterns, stability and efficiency. Mishra M, Tamhane VA, Khandelwal N, Kulkarni MJ, Gupta VS, Giri AP. Proteomics. 2010 Aug;10(15):2845-57. 31. Discovery of rifampicin as a new anti-glycating compound by matrix-assisted laser desorption / ionization mass spectrometry-based insulin glycation assay. Golegaonkar SB, Bhonsle HS, Boppana R, Kulkarni MJ*. Eur J Mass Spectrom (Chichester, Eng). 2010;16(2):221-6. 32. LC-UV and LC-MS evaluation of stress degradation behaviour of tenatoprazole. Mahadik MV, Kulkarni MJ, Dhaneshwar SR. Journal of Pharmaceutical and Biomedical Analysis 2009:, 50: 787–793. 33. Column liquid chromatography-ultraviolet and column liquid chromatography/mass spectrometry evaluation of stress degradation behavior of escitalopram oxalate. Dhaneshwar SR, Mahadik MV, Kulkarni MJ. J AOAC Int. 2009 Jan-Feb;92(1):138-47. 34. Albumin competitively inhibits glycation of less abundant proteins. Bhonsle HS, Singh SK, Srivastava G, Boppana R, Kulkarni MJ*. Protein Pept Lett. 2008;15(7):663-7 35. Study of papain-cystatin interaction by intensity fading MALDI-TOF-MS. Shabab M, Kulkarni MJ*, Khan MI. Protein J. 2008 Jan;27(1):7-12. 36. Characterization of the proteins of bacterial strain isolated from contaminated site involved in heavy metal resistance--a proteomic approach. Bar C, Patil R, Doshi J, Kulkarni MJ*, Gade WN. J Biotechnol. 2007 Feb 20;128(3):444-51. Epub 2006 Nov 24. 37. An attempt at taxonomical characterization of some Rhizobial species by intact cell MALDI mass spectrometry. Prabhu RR, Kulkarni MJ*, Parshurami V, Santhakumari V, Paranjape S World J Microbiol Biotechnology. 2007; 23:177-185. 38. Intact cell matrix-assisted laser desorption/ionization mass spectrometry as a tool to screen drugs in vivo for regulation of protein expression. Kulkarni MJ*, Vinod VP, Umasankar PK, Patole MS, Rao M. Rapid Commun Mass Spectrom. 2006;20(18):2769-72. 39. Differential protein expression in human gliomas and molecular insights.Chumbalkar VC, Subhashini C, Dhople VM, Sundaram CS, Jagannadham MV, Kumar KN, Srinivas PN, Mythili R, Rao MK, Kulkarni MJ, Hegde S, Hegde AS, Samual C, Santosh V, Singh L, Sirdeshmukh R. Proteomics. 2005 Mar;5(4):1167-77. 40. Cytokinin oxidase activity and cytokinin content in roots of sunflower under water stress. Manju RV, Kulkarni MJ, Prasad TG, Sudarshana L, Sashidhar VR. Indian J Exp Biol. 2001 Aug;39(8):786-92. 41. A novel colorimetric assay and activity staining for cytokinin oxidase based on the copper amine oxidase property of the enzyme. Kulkarni MJ, Theerthaprasad D, Sudharshana L, Prasad TG, Sashidhar VR. Phytochem Anal. 2001 May-Jun;12(3):180-4. 42. Genotypic variation in ‘early warning signals’ from roots in drying soil : Intrinsic differences in ABA synthesizing capacity rather than root density determines total ABA ‘message’ in cowpea (Vigna ungiculata L.) in top dry zone. Kulkarni MJ, Prasad TG and Sashidhar VR Annals of Applied Biology (UK). (2000); 136: 267-272. 43. Should Plants keep their canopy ‘cool’ or allow it to grow ‘warm’ under stress: It’s a Hobsons choice and plants survive by doing a balancing act. Sashidhar, VR., Ankegowda SJ., Kulkarni MJ, Srinivas MN., Prasad TG. and Devendra, R, Current Science(2000). 78: 786-789. 44. Biochemical and histological changes associated with downy mildew infection in sunflower. Prashanth Kumar BR., Kulkarni MJ, Veena Rao BN., Chandrika K, Balakrishna G. Helia,2000; 23: 1-18. 45. Slower-chemical or faster electrical signalling under stress in plants: Is it the hare and tortoise story of slower signal winning the race? Rekha G., Sudarshana L., Prasad TG, Kulkarni MJ and Sashidhar VR., Current Science. (1996). 71 : 284-289. Patents Methods of producing and developing cold temperature tolerant plants, seed and crops (co-author at Monsanto Research center Bangalore). WO 2007/ 112122 A2